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The design and characterization of a lactate biosensor and its application to the determination of this analyte in wine and beer are described. The biosensor is developed through the immobilization of lactate oxidase (LOx) using two different strategies including direct adsorption and covalent binding. The characterization of the resulting lactate oxidase monolayers was performed in aqueous phosphate buffer solutions using atomic force microscopy (AFM) and quartz crystal microbalance (QCM) techniques. In presence of lactate and using hydroxymethylferrocene as a redox mediator, biosensors obtained by either direct adsorption or by covalent binding exhibit a clear electrocatalytic activity, and lactate could be determined amperometrically at 300 mV versus SSCE. Results obtained under these conditions give a linear current response versus lactate concentration up to 0.3 mM, with a detection limit of 10 muM of lactate and a sensitivity of 0.77 ± 0.08 muA mM-1. Finally, biosensors were applied to the determination of lactate in wine and beer. The results obtained are in good agreement with those obtained by a well-established enzymatic-spectrophotometric assay kit.

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Reference:
Iron Catalysis in Organic Synthesis | Chemical Reviews,
Iron Catalysis in Organic Synthesis: A Critical Assessment of What It Takes To Make This Base Metal a Multitasking Champion

 

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In electrochemical systems imperfect thermostating inevitably leads to the presence of bulk convective flows. As recognised by Nernst [Z. Phys. Chem., 1904, 52] damping of these bulk convective flows next to a solid surface, or at the electrode, leads to diffusional mass transport predominating locally. This work questions the exclusivity of diffusional transport and provides hitherto unexplored physical insights into how thermally induced flows in bulk solution can, on both macro- and microelectrodes, influence a voltammetric measurement. Imperfect thermostating results in flows in the bulk solution which are predicted and here expeimentally shown to be of the order of 100 mum s-1. Here we show that even in the absence of natural convective flows induced by the electrochemical reaction itself, this thermally induced bulk convection can significantly affect the voltammetric response. First, evaporative losses from an open electrochemical cell can be sufficient to produce convective flows that can alter the electrochemical response. Second, electrodes with various sizes and geometries have been investigated and experimental results evidence that the sensitivity of an electrode to these flows in bulk solution is to a large extent controlled by the size of the surrounding non-conductive supporting substrate used to insulate parts of the electrode.

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Reference:
Iron Catalysis in Organic Synthesis | Chemical Reviews,
Iron Catalysis in Organic Synthesis: A Critical Assessment of What It Takes To Make This Base Metal a Multitasking Champion

 

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Application of 1273-86-5, hemistry, like all the natural sciences, begins with the direct observation of nature— in this case, of matter. In a document type is Article, molecular formula is C11H3FeO, molecular weight is 206.99, and a compound is mentioned, 1273-86-5, Ferrocenemethanol, introducing its new discovery.

Abstract: Regioselective synthesis of bis-ferrocenylpyrazole derivatives in biphasic aquatic?organic system under catalysis with HBF4 was carried out. The regioselectivity of these reactions depending on the electronic effects of substituents in pyrazole moiety was studied. Graphical abstract: [Figure not available: see fulltext.]

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Reference:
Iron Catalysis in Organic Synthesis | Chemical Reviews,
Iron Catalysis in Organic Synthesis: A Critical Assessment of What It Takes To Make This Base Metal a Multitasking Champion

 

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The viability and activity of HeLa cells were probed using scanning electrochemical microscopy (SECM). The feedback generated by HeLa cells during scanning depends on the electrochemical mediator. Living HeLa cells generated positive feedback when ferrocene methanol (FcMeOH) was oxidized at the tip, showing that the cells reduced FcMeOH+. The positive feedback with FcMeOH changed to negative feedback when the HeLa cells were exposed to toxic treatments, i.e. CN- or UVC radiation, suggesting that FcMeOH+ reduction can be used to monitor cell activity. Living HeLa cells also accumulate FcMeOH after exposure times of a few h, but the presence of mM concentrations of FcMeOH has no apparent effect on the cell viability. The effect of Ag+ (known to be toxic to bacteria at the 10 muM level) on HeLa cells was probed using the FcMeOH as an indicator. The activity of the HeLa cells was not affected in a culture medium containing Ag+ up to 10 mM. The uptake of Ag+ by living and dead HeLa cells was small and nearly the same, indicating that even at high Ag+ concentrations in the culture medium, only a small amount of Ag+ is accumulated within the cells.

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Reference:
Iron Catalysis in Organic Synthesis | Chemical Reviews,
Iron Catalysis in Organic Synthesis: A Critical Assessment of What It Takes To Make This Base Metal a Multitasking Champion

 

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Chemistry is a science major with cience and engineering. The main research directions are preparation and modification of special coatings, and research on the structure and performance of functional materials. In a patent, 1273-86-5, name is Ferrocenemethanol, introducing its new discovery. Application In Synthesis of Ferrocenemethanol

The invention discloses a ferrocene-containing base element of the compound and its preparation method and application, the ferrocene-containing base element of the structural formula of the compound is shown as formula I: (by machine translation)

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Reference:
Iron Catalysis in Organic Synthesis | Chemical Reviews,
Iron Catalysis in Organic Synthesis: A Critical Assessment of What It Takes To Make This Base Metal a Multitasking Champion

 

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The reactant in an enzyme-catalyzed reaction is called a substrate. Enzyme inhibitors cause a decrease in the reaction rate of an enzyme-catalyzed reaction. Quality Control of Ferrocenemethanol. In heterogeneous catalysis, catalysts provide a surface to which reactants bind in a process of adsorption. Introducing a new discovery about 1273-86-5, Name is Ferrocenemethanol

Neuronal activity and brain glucose metabolism are tightly coupled, where triggered neurotransmission leads to a higher demand for glucose. To better understand the regulation of neuronal activity and its relation to high-speed metabolism, development of analytical tools that can temporally resolve the transients of vesicular neurotransmitter release and fluctuations of metabolites such as glucose in the local vicinity of the activated neurons is needed. Here we present an amperometric biosensor design for rapid co-detection of glucose and the neurotransmitter dopamine. The sensor is based on the immobilization of an ultra-thin layer of glucose oxidase on to a gold-nanoparticle-covered carbon fiber microelectrode. Our electrode, by altering the potential applied at the sensor surface, allows for the high-speed recording of both glucose and dopamine. We demonstrate that, even though glucose is electrochemically detected indirectly through the enzymatic product and the electroactive dopamine is sensed directly, when exposing the sensor surface to a mixture of the two analytes, fluctuations in glucose and dopamine concentrations can be visualized with similar speed and at a millisecond time scale. Hence, by minimizing the enzyme coating thickness at the sensor surface, dual detection of glucose and dopamine can be realized at the same sensor surface and at time scales necessary for monitoring fast metabolic alterations during neurotransmission.

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Reference:
Iron Catalysis in Organic Synthesis | Chemical Reviews,
Iron Catalysis in Organic Synthesis: A Critical Assessment of What It Takes To Make This Base Metal a Multitasking Champion

 

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A series of 1H-1,2,3-triazole linked Ferrocenyl-methoxy-methyl-Isatin conjugates was synthesized and assayed for their anti-proliferative activities against estrogen-responsive as well as estrogen non-responsive cell lines. The non-cytotoxic conjugate 7l, with an optimum combination of octyl chain as spacer and methyl-substituent at the C-5 position of isatin, proved to be a promising hit with an IC50 value of 14.62 muM against MCF-7 and 79.63 muM against MDA-MB-231 cells, respectively. The observed anti-proliferative activities of active conjugates were further corroborated via docking studies carried out on estrogen receptor subtypes alpha and beta.

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Reference:
Iron Catalysis in Organic Synthesis | Chemical Reviews,
Iron Catalysis in Organic Synthesis: A Critical Assessment of What It Takes To Make This Base Metal a Multitasking Champion

 

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Multilayer films made of poly(diallyldimethylammonium) (PDDA), gold nanoparticles (AuNP) of 10 nm diameter and lactate oxidase (LOx), were built for the first time, by layer-by-layer (LBL) assembly technique onto both quartz and gold substrates. The surface plasmon resonance (SPR) band of AuNP was used for monitoring the film growth and adsorption kinetics on PDDA monolayer supported onto quartz substrate. In the case of polycrystalline gold surfaces, electrochemical characterization was performed by means of cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The latter system was used as bioelectrode for L-lactate quantification, showing a short time response (<10 s) with high sensitivity of 63 ± 3 muA mM?1cm?2, detection limit of 0.47 muM and linear range between 0.001 mM and 0.25 mM. This bioelectrode was also tested for L-lactate analysis in yogurt and fermented milk, and it was checked that typical interfering as glucose and citric, acetic and ascorbic acids do not play a role. Present results indicate that self-assembled multilayers made of PDDA/AuNP/LOx are suitable thin films for electroanalytical determination of L-lactate in real samples. Future efforts will undeniably focus on the diversification of the new catalytic transformations. We’ll also look at important developments of the role of 1273-86-5, and how the biochemistry of the body works.Electric Literature of 1273-86-5

Reference:
Iron Catalysis in Organic Synthesis | Chemical Reviews,
Iron Catalysis in Organic Synthesis: A Critical Assessment of What It Takes To Make This Base Metal a Multitasking Champion

 

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Different modes of scanning electrochemical mapping (SECM) such as surface generation/tip collection (SG/TC), amperometry, AC-SECM and potentiometry were employed to characterize the active/passive domains, hydrogen gas (H2) evolution and local pH on a corroding surface of AZ31 in simulated biological fluid (SBF). It was found that the main domains of H2 evolution are associated with lower insulating properties of the surface as well as higher local pH. The near surface pH was found to be highly alkaline indicating that, even in a buffered solution such as SBF, the local pH on a corroding AZ31 surface can be significantly different to the bulk pH.

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Reference:
Iron Catalysis in Organic Synthesis | Chemical Reviews,
Iron Catalysis in Organic Synthesis: A Critical Assessment of What It Takes To Make This Base Metal a Multitasking Champion

 

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The result showed that such a combination of chemo- and biocatalysis improved the catalytic yield more than two times compared with that of sole metal catalysis. We will look forword to the important role of 1273-86-5, and how the biochemistry of the body works.Electric Literature of 1273-86-5

Electric Literature of 1273-86-5, In heterogeneous catalysis, catalysts provide a surface to which reactants bind in a process of adsorption. In homogeneous catalysis, catalysts are in the same phase as the reactants. In a document type is Article, and a compound is mentioned, 1273-86-5, name is Ferrocenemethanol, introducing its new discovery.

We investigate the Faradaic currents of ferrocene species at an electrode-membrane-electrode system. The membrane electrodes system is fabricated by physical sputtering of conductive metal on both sides of a nanoporous alumina membrane. The metal coatings function as working electrodes by connection to a bipotentiostat and reference/counter electrodes in the sample solution. Collection and shielding efficiency studies are carried out at this membrane electrodes system to measure mobility values of charged ferrocene species within nanochannels of alumina membrane. Results indicated high selective electrode response of up to 16 times for positively charged (dimethylaminomethyl)ferrocene (FcN), and neutral ferrocenemethanol (FcMeOH), compared to the negatively charged ferrocenecarboxylic acid (FcCOOH) when the receiver electrode was maintained at negative potentials of 0.5-1.0 V relative to the feed electrode. This is a report on the use of an electrode-membrane-electrode system to achieve selective response towards differently charged redox species, under the condition of high electrolyte concentration (0.1 M, pH 7.0 phosphate buffer).

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Reference:
Iron Catalysis in Organic Synthesis | Chemical Reviews,
Iron Catalysis in Organic Synthesis: A Critical Assessment of What It Takes To Make This Base Metal a Multitasking Champion