Related Products of 1273-86-5, The reactant in an enzyme-catalyzed reaction is called a substrate. Enzyme inhibitors cause a decrease in the reaction rate of an enzyme-catalyzed reaction. 1273-86-5, Name is Ferrocenemethanol, molecular weight is 206.99. belongs to iron-catalyst compound, In an Article£¬once mentioned of 1273-86-5
Cholesterol oxidase modified gold electrodes as bioanalytical devices
Cholesterol oxidase (ChOx) has been immobilized by direct adsorption on gold electrodes. The resulting ChOx monolayers have been characterized using atomic force microscopy (AFM) under liquid conditions and quartz crystal microbalance (QCM) techniques. The immobilized enzyme retains its catalytic activity, thus spatially resolved mapping of enzymatic activity has been carried out using scanning electrochemical microscopy (SECM). The replacement, in the enzymatic reaction, of the natural electron acceptor (O2) by an artificial mediator has been also evaluated, in particular, hydroxymethylferrocene (HMF), thionin, nile blue and azure A, have been studied as electron acceptors for reduced ChOx. In addition, the influence of the low cholesterol solubility on the experimental conditions using redox mediators was also discussed. Finally, the response of the enzymatic electrode to varying cholesterol concentrations has been obtained by measuring directly the H2O2 generated in the enzymatic reaction. Cholesterol can be determined amperometrically at +0.5 V (versus SSCE) with a detection limit of 60 muM and a sensitivity of 0.13 muA mM-1.
Cholesterol oxidase modified gold electrodes as bioanalytical devices
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Reference£º
Iron Catalysis in Organic Synthesis | Chemical Reviews,
Iron Catalysis in Organic Synthesis: A Critical Assessment of What It Takes To Make This Base Metal a Multitasking Champion